The double-amputee Olympian and Paralympian "Blade Runner" will be seeking bail at the hearing at a court in the capital Pretoria which is expected to reveal more about what happened the night his lover Reeva Steenkamp was killed.
Steenkamp, 29, who was shot four times in the early hours of Thursday by a 9mm pistol owned by the sprinter, is due to be laid to rest at a private ceremony in her hometown of Port Elizabeth later Tuesday.
Pistorius was transferred to the court in the early hours of Tuesday, according to local media reports.
Pistorius's family has insisted that the evidence will refute "any possibility of a premeditated murder or indeed any murder at all".
Defence lawyers are expected to argue that there are "exceptional circumstances" for 26-year-old Pistorius to be freed pending trial.
As a result, more details will likely emerge of what happened during the Valentine's Day shooting at Pistorius's upmarket home in a gated estate in Pretoria.
The state, arguing premeditated murder, will oppose bail.
Both sides have called in heavyweight lawyers, setting the scene for a massive legal showdown.
Unconfirmed reports have suggested Pistorius mistook Steenkamp for an intruder, while other reports said she was beaten with a cricket bat before being shot multiple times.
In an interview published on Monday, Steenkamp's mother described Reeva's death as "horrendous."
"Why my little girl? Why did this happen? Why did he do this?" June Steenkamp told the Times of South Africa.
"She had so much of herself to give and now all that is gone. Just like that, she is gone... In the blink of an eye and a single breath, the most beautiful person who ever lived is no longer here."
Steenkamp, who had been going out with Pistorius since late last year, will be laid to rest in a private ceremony at a Port Elizabeth crematorium with an on-site chapel that holds less than 100 mourners.
"The memorial itself inside the chapel is family and friends and then there will be a statement afterwards for the media," her uncle Michael Steenkamp told AFP.
The family bore the runner no grudge but wanted clarity about Steenkamp's death.
"The family haven't got that animosity or hatred or anything like that but questions, and we realise that it's going to come out," he said.
Sharon Steenkamp, her cousin, told local newspaper The Herald that none of the family at Steenkamp's parents home had met Pistorius.
"No, we are not even thinking about Oscar. All our focus is on Reeva, her life and what has happened to her. She never had an enemy in the world and was a super person," she said.
On Saturday a celebrity television show aired haunting footage of Reeva Steenkamp speaking about the need to leave a positive mark on life, words laden with poignancy after her death.
"Not just your journey in life, but the way that you go out and make your exit is so important, you have either made an impact in a positive way or a negative way," she said.
Pistorius, who broke down in sobs during his first court appearance on Friday, has built up a powerful team of lawyers, medical specialists and public relations experts for his defence.
Stuart Higgins, a former editor of British tabloid The Sun, whose lengthy list of clients includes British Airways, Chelsea FC and Manchester United football club, has taken over his public relations.
One of the lawyers, Kenny Oldwage, defended the driver in a 2010 accident that killed former president Nelson Mandela's great-grandchild Zenani. The driver was acquitted.
A global inspirational figure, Pistorius was the first double amputee to compete against able-bodied athletes in the 2012 London Olympic Games. His nickname was inspired by his custom-built carbon-fibre running blades.
The Johannesburg-born runner had both legs amputated below the knee when he was 11 months old after being born without lower leg bones.
The killing has put his career on hold, forcing him to cancel races in Australia, Brazil, Britain and the United States between March and May.